Kev Tshawb Fawb Lub Hom Phiaj thiab Cov Ntsiab Lus Tseem Ceeb
lub hom phiaj
Hauv kev kho mob ntawm tes thiab noob caj noob ces (CGT), Kev Tshaj Tawm -Kev Ntsuas Tus Kab Mob (RCV) yog qhov tseem ceeb ntawm kev tswj hwm thiab ntsuas kev nyab xeeb. Qhov kev sim no kuaj pom kev rov ua dua tshiab- cov kab mob muaj peev xwm uas tuaj yeem tshwm sim thaum tsim khoom los ntawm kev sib xyaw ua ke lossis hloov pauv, yog li tiv thaiv kev pheej hmoo xws li kev tiv thaiv kab mob lossis qog nqaij hlav hauv cov neeg mob.
Txhais
Viral vectors nquag siv hauv CGT-xws li AAV, lentivirus, retrovirus, thiab adenovirus- feem ntau yog replication- tsis zoo los ntawm kev tsim. Txawm li cas los xij, thaum lub sij hawm tsim khoom, lawv tuaj yeem tau txais kev ua kom muaj peev xwm los ntawm homologous lossis tsis yog -homologous recombination, lossis hloov pauv hloov pauv. Qhov no ua rau muaj kev rov ua dua tshiab- muaj peev xwm hloov pauv xws li rcAAV, RCL, RCR, thiab RCA, uas tuaj yeem rov ua dua tus kheej thiab nyob twj ywm kis tau raws li kev tso cai.
Lub Hom Phiaj Tseem Ceeb rau Kev Xeem
Hom RCV Risk Scenarios los ntawm Vector Hom:
|
Hom Vector |
RCV Type |
Yam Risk Scenarios |
|
Adeno-sociated virus (AAV) |
Replication -competent AAV (rcAAV) |
Thaum muaj tus kab mob pab, rcAAV tuaj yeem rov ua dua, muaj peev xwm ua rau lub cev tsis muaj zog thiab ua rau muaj mob qog noj ntshav. |
|
Lentiviral vector (LV) |
Kev rov ua dua -Lentivirus (RCL) muaj peev xwm |
HIV-1-derived vectors tuaj yeem hloov pauv hloov pauv, rov ua kom muaj peev xwm ua kom muaj kab mob. |
|
Retroviral vector (RV) |
Kev rov ua dua -tus neeg muaj peev xwm retrovirus (RCR) |
Kev hloov pauv hloov pauv tuaj yeem ua rau muaj qhov txawv txav ntawm cov cell proliferation thiab insertional mutagenesis. |
|
Adenovirus (AdV) |
Kev rov ua dua - muaj peev xwm adenovirus (RCA) |
ΔE1-cov vectors uas tau muab tshem tawm tuaj yeem rov ua dua nrog E1 cov kab ke hauv cov neeg tsim khoom (piv txwv li, HEK293), rov tsim cov tsiaj qus- hom replication-competent virus. |
Qhov zoo ntawm Peb RCV nrhiav tau Platform
HyQure Biotech tau tsim muaj peev xwm txheeb xyuas tau zoo rau kev rov ua dua -cov kab mob muaj peev xwm (RCVs), suav nrog rcAAV, RCL, RCR, thiab RCA. Peb qhov kev xeem rcAAV yog CNAS- ntawv pov thawj. Peb ntau -RCV nrhiav kom pom qhov system muab peb qhov zoo tshaj plaws - qhov tseeb, ceev, thiab kev tswj hwm. Txoj kev kab lis kev cai ntawm tes ua haujlwm raws li tus qauv kub; ua ke nrog kev tshawb pom qhov kawg, nws tshwj xeeb txheeb xyuas cov kab mob RCVs, ncaj qha hais txog kev nyab xeeb kev nyab xeeb thaum ua tau raws li tag nrho cov kev cai xeem.