NGS-enabled RNAkev kho mob:puv -kev tshawb fawb thiab kev tsim kho thiab kev lag luam
NGS solution is aKev paub txog kev txheeb xyuas qhov tseeb tsim los rau kev txiav -ntug RNA tshuaj xws li mRNA therapy, siRNA therapy, thiab RNA kho kho. Nws tsom mus rau qhov tseeb, kev ncaj ncees, thiab kev ruaj ntseg ntawm caj ces ntawm RNA molecules. Los ntawm peb lub thev naus laus zis thev naus laus zis-mRNA sequencing, ultra- tsis tshua muaj kev tshuaj ntsuam xyuas, thiab RNA kev ntsuam xyuas kev ncaj ncees- nws npog tag nrho cov txheej txheem tswj kev ua tau zoo ntawm RNA kev kho mob los ntawm kev tshawb fawb thiab kev loj hlob thaum ntxov, kev tsim tawm mus rau kev lag luam. Qhov no pab cov tuam txhab kov yeej kev tsis sib haum xeeb hauv kev tsim tshuaj RNA, ua tau raws li kev tswj hwm thoob ntiaj teb, thiab ua kom cov khoom muaj kev nyab xeeb thiab ua tau zoo.
Kev simCov ntsiab lus kev pabcuam
• Test cov ntsiab lus:Pull-length sequence txiav txim siab: Siv high-fidelity RNA-seq thev naus laus zis, tag nrho-length mRNA sequence yog soj ntsuam tag nrho los txheeb xyuas qhov tseeb ntawm cov coding thiab uncoding (UTR) sequences thiab tshuaj ntsuam xyuas cov kab ke sib txawv, qhov sib txawv ntawm qhov sib txawv, xws li kev sib txawv ntawm qhov sib txawv;
•Isomer tsom xam: Rau cov mRNA cov molecules complex, txheeb xyuas cov hom thiab proportions ntawm lwm splicing isomers thiab ntsuam xyuas lawv cov kev cuam tshuam rau cov tshuaj muaj nuj nqi;
Kev txheeb xyuas qhov sib xws: Sib piv mRNA ib ntus los ntawm cov khoom sib txawv ntawm cov khoom sib txawv thiab nyob rau hauv cov xwm txheej sib txawv los xyuas kom muaj kev ruaj ntseg ntawm cov khoom siv thiab hauv lub txee lub neej;
Cov txiaj ntsig zoo: Kev sib npaug ntawm cov kev pab cuam> 99%, qhov sib npaug ntawm qhov tseeb Ntau dua lossis sib npaug li 99%, thiab tuaj yeem ntes tau qis - ntau yam sib txawv.
• Kev kuaj pom muaj peev xwm:Nws tuaj yeem txheeb xyuas qhov txawv ntawm qhov tsawg kawg nkaus uas tsis tshua muaj zaus (xws li qhov tsis tshua muaj qhov hloov pauv, chimeric sequences, thiab degradation-txog txawv).
• Kev pab txhawb nqa:Lub kaw lus siv ultra- sib sib zog nqus sib sib zog nqus ua ke nrog cov tswv ntawm bioinformatics algorithms los lim cov suab nrov tom qab thiab txhim kho qhov tseeb ntawm qhov tsis tshua muaj qhov sib txawv.
Kev ncaj ncees ntawm RNA molecules ncaj qha txiav txim siab lawv cov kev txhais lus thiab cov txiaj ntsig kho mob, thiab yog qhov qhia tau zoo rau kev tswj hwm kev kho RNA.
Daim ntawv qhia txog -length integrity verification:Tag nrho -length ratio ntawm mRNA/siRNA molecules yog soj ntsuam los ntawm capillary electrophoresis thiab RNA-seq kev ntsuam xyuas los txheeb xyuas cov khoom truncated thiab degradation;
Secondary qauv stability tsom xam:Ib qho RNA folding twv twv algorithm ua ke nrog kev sim tshuaj ntsuam xyuas tau siv los ntsuas qhov ncaj ncees ntawm RNA molecule tus qauv thib ob, zam kev cuam tshuam ntawm cov qauv kev txawv txav ntawm kev xa khoom thiab kev ua haujlwm;
Scope ntawm Daim Ntawv Thov Kev Xeem
mRNA kev kho mob
Cov tshuaj tiv thaiv kab mob COVID-19 mRNA, tshuaj tiv thaiv kab mob qog noj ntshav mRNA, tshuaj tiv thaiv protein hloov mRNA, thiab lwm yam.
Kev kho mob me me RNA
siRNA tshuaj, miRNA mimics/inhibitors, thiab lwm yam.;
RNA kho kho kho
CRISPR-Cas13- kho RNA kho kho tshuaj kho mob, ADAR enzyme-mediated RNA hloov kho kho, thiab lwm yam.
Lwm cov kev kho mob RNA suav nrog
Cov tshuaj RNA ncig, ntev tsis yog -coding RNA (lncRNA) kev kho mob, thiab cov tshuaj RNA aptamer.