Kev txiav txim siab tsim nyog rau plaub txoj hauv kev
Bioassay
Txheeb xyuas cov hom phiaj xws li cov kab mob antigens thiab cov proteins tshwj xeeb.
Optimize cov tshuaj tiv thaiv kab mob; teem lub hom phiaj rau linear range thiab limit of detection (LOD).
ELISA (Enzyme-Txuas Kev Ntsuas Immunosorbent)
Tshuaj ntsuam xyuas cov tshuaj tiv thaiv kab mob tshwj xeeb (kev ntes thiab kuaj cov tshuaj tiv thaiv).
Optimize txheej concentration, thaiv tsis pub, thiab incubation lub sij hawm tsis.
qPCR (Real -Time Quantitative PCR)
Tsim tshwj xeeb primers/probes kom tsis txhob hla -reactivity.
Kho cov tshuaj tiv thaiv tsis xws li annealing kub thiab ncua sij hawm.
Xaiv cov noob siv los xyuas kom meej cov ntaub ntawv normalization.
Cell-Raws li kev soj ntsuam
Xaiv cov cell tsim nyog; txiav txim siab seeding ceev thiab kab lis kev cai ntev.
Tsim cov txheej txheem txhais lus, xws li fluorescence intensity thresholds thiab cytopathic effect (CPE) scoring.
Optimization Workflow
Siv ib zaug ib zaug -factor lossis orthogonal thwmsim los ntsuas qhov tseem ceeb xws li cov tshuaj tiv thaiv kub thiab cov khoom ntim reagent. Los ntawm kev kaw - lub voj optimization txheej txheem ntawm "kev hloov pauv parameter → kev txheeb xyuas qhov tshwm sim → kev txheeb xyuas qhov sib txawv," txhim kho qhov tseeb, qhov tseeb, thiab muaj zog ntawm txoj kev.
Core Zoo
NO.1
Npog plaub hom kev tshawb nrhiav, sib xws nrog ntau yam kev kuaj lom.
NO.2
Customizable daws teeb meem raws li qhov sib txawv nrhiav kom tau qhov tseeb.
NO.3
Kaw - txheej txheem kev ua kom zoo dua ua kom txoj kev ruaj khov thiab kev ntseeg tau.