Kev nyab xeeb kev nyab xeeb hauv kev tshawb nrhiav kab mob vector thiab tsim khoom
Viral vector detection yog ib qho kev daws teeb meem tshwj xeeb uas tsim los rau biomedical teb xws li kev kho noob thiab kev kho ntawm tes. Kev vam khom siab thiab muaj txiaj ntsig zoo ntawm qhov txuas ntxiv ntawm - tiam sequencing (NGS) thev naus laus zis, nws muab kev ntsuam xyuas kev nyab xeeb thiab cov cwj pwm ntawm caj ces txhawb rau kev tshawb fawb, kev tsim kho, kev tsim khoom thiab kev tswj xyuas zoo ntawm vectors xws li AAV, lentiviruses thiab cov kab mob oncolytic, pab cov tuam txhab thiab cov koom haum tshawb fawb kom dhau los ua cov khoom lag luam.
Kev pab them nqi ntawm mainstream viral vector hom
Peb tsom mus rau peb qhov tseem ceeb ntawm cov kab mob viral thiab muab cov kev pabcuam kuaj mob:
Targeting recombinant AAV vectors (xws li serotypes AAV1-13), haum rau kev siv xws li hauv vivo xa thiab hauv vitro cell transduction hauv kev kho noob;
Npog HIV- muab tau cov kab mob lentiviruses thiab kev sib xyaw ua ke / tsis yog- cov kab mob lentiviral vectors kom tau raws li qhov kev xav tau ntawm kev kho mob ntawm tes (xws li CAR-T), gene kho thiab lwm yam;
suav nrog adenoviruses, herpesviruses, poxviruses, thiab lwm yam oncolytic vectors, uas txhawb kev nyab xeeb thiab kev ntsuas kev ua tau zoo hauv kev txhim kho cov tshuaj tiv thaiv kab mob.
Tom ntej no -Generation Sequencing (NGS) Technology Enables Precision Detection
Kev siv kev lag luam- ua tus thawj coj NGS thev naus laus zis platform, ua ke nrog cov kev tsim kho tsev qiv ntawv kho kom haum thiab bioinformatics tsom xam cov haujlwm ua haujlwm, peb ua tiav kev kuaj pom tus kab mob vectors:
Kev tiv thaiv siab: Kev ntes cov vector genome thiab cov ntaub ntawv qhia txog kev ua qias tuaj ntawm exogenous
Kev tshawb pom tseeb ntawm ntau qhov ntsuas: Ib txhij ua tiav ntau qhov ntsuas xws li kev tshuaj ntsuam kab mob exogenous thiab kev txheeb xyuas qhov sib txawv;
Cov txheej txheem ua tau zoo: Los ntawm cov qauv txais tos, nucleic acid extraction, tsev qiv ntawv kev tsim kho rau cov ntaub ntawv txheeb xyuas, tag nrho cov txheej txheem yog tus qauv, ua kom lub voj voog sim.
Dual - qhov kev pab them nqi ntawm kev soj ntsuam kev nyab xeeb thiab kev txheeb xyuas tus yam ntxwv
Kev tshuaj xyuas ntau ntawm cov kab mob exogenous yog ua rau cov khoom siv vector thiab cov plasmids tseem ceeb kom ntseeg tau tias muaj kev nyab xeeb ntawm cov khoom siv kho mob.
• Paub txog kev tshawb nrhiav exogenous: npog cov kab mob xws li mycoplasma, kab mob, fungi, thiab endogenous retroviruses (ERV);
• Unknown exogenous sequence screening: siv metagenomic sequencing thev naus laus zis los tshawb pom cov kab mob uas tsis paub txog lossis cov kab mob sib kis;
• Hla-kev tshuaj ntsuam kab mob sib kis: Txheeb xyuas tus ntoo khaub lig- sib kis ntawm cov khoom sib txawv ntawm cov neeg nqa khoom thiab nruab nrab ntawm cov plasmids thiab cov nqa khoom kom ntseeg tau tias cov khoom huv si.
Kev piav qhia ntawm caj ces tus yam ntxwv ntawm tus kab mob vectors thiab plasmids tseem ceeb ua kom cov khoom sib xws thiab ruaj khov.
• Kev txheeb xyuas qhov tseeb ntawm Vector genome: Tshawb xyuas tag nrho - ntev vector kab ke, qhov tseeb ntawm lub hom phiaj gene insertion , thiab cov ntaub ntawv hloov pauv ;
• Kev tshuaj ntsuam xyuas thiab kev txheeb xyuas Plasmid: Rau cov khoom tseem ceeb plasmids (xws li ntim cov plasmids thiab hloov plasmids), ua raws li kev txheeb xyuas qhov tseeb thiab kev tshuaj ntsuam kab mob;
• Kev ntsuam xyuas kev ruaj ntseg ntawm caj ces: Saib xyuas qhov kev hloov pauv caj ces ntawm cov vectors hauv cov khoom sib txawv los xyuas kom meej qhov sib xws ntawm cov khoom batch.
Kev koom ua ke tag nrho cov txheej txheem los ntawm R & D mus rau ntau lawm
Vector tsim optimization, plasmid tshuaj ntsuam xyuas thiab kev txheeb xyuas, thiab kev ntsuam xyuas kev nyab xeeb thaum ntxov ua lub hauv paus rau kev sim tom ntej;
batch zoo tswj, txheej txheem optimization kev txhawb nqa, thiab saib xyuas cov pa phem sab nraud kom ntseeg tau tias ua raws li cov txheej txheem ntau lawm;
Kev soj ntsuam kev nyab xeeb thiab cov ntaub ntawv qhia txog caj ces uas ua raws li GMP cov qauv thiab ua tau raws li kev cai qhia txog kev cai;
Kev ntsuam xyuas batch, kev tshuaj xyuas kev tso tawm zoo, thiab kev ruaj ntseg ntev -
Kev Pabcuam Zoo - Kev tshaj lij, Ua tau zoo, thiab kho tau
Kev paub dav dav
Tsom ntsoov rau thaj tsam ntawm kev sim tshuaj biomedical tau ntau xyoo, sib sau ua ke ntau tus kab mob vector kuaj mob, yoog tau rau cov ntawv thov sib txawv;
Kev coj noj coj ua
Tsis tu ncua iterating NGS nrhiav kom tau technology thiab tsom xam algorithms los xyuas kom meej qhov tseeb thiab kev ntseeg tau ntawm nrhiav tau cov txiaj ntsig;
Kev Pabcuam Ua Raws Cai
Cov txheej txheem xeem ua raws li GLP / GMP cov qauv, thiab cov ntawv ceeb toom ua tau raws li cov kev cai ntawm cov koom haum tswj hwm xws li NMPA, FDA, thiab EMA;
Cov kev pabcuam customized
Kev ntsuam xyuas cov kev daws teeb meem tuaj yeem hloov kho raws li cov neeg siv khoom xav tau, thiab tus kheej cov ntaub ntawv txheeb xyuas thiab kev txhais lus txhawb nqa tuaj yeem muab.