Xauv cov yam ntxwv tseem ceeb ntawm cov kab ntawm tes thiab tso lub hauv paus ruaj khov rau kev lag luam
Tom ntej no -generation sequencing (NGS) technology muabKev nthuav qhia xov tooj ntawm tes tsom xam daws teeb meem nrog nws cov peev txheej sib sib zog nqus, ua kom muaj kev tshawb fawb txog cov yam ntxwv ntawm caj ces, cim cim, thiab kev nyab xeeb keeb kwm yav dhau ntawm ntau cov kab ntawm tes. Qhov no muab cov ntaub ntawv raug txhawb rau kev tshuaj ntsuam kab ntawm tes, kev siv tau, kev siv, thiab kev tswj kom zoo.
Tag nrho -genome NGSua ke
Los ntawm kev ua haujlwm siab - qhov tob tag nrho- genome sequencing thev naus laus zis, ua ke nrog cov txheej txheem tshawb fawb bioinformatics siab heev, peb tuaj yeem ua tiav qhov kev nkag siab zoo thiab meej ntawm cov kab ntawm tes:
Siab - qhov tob
Sequencing qhov tob tuaj yeem ncav cuag Ntau dua lossis sib npaug li 50 ×, kom ntseeg tau tias kev ntes cov ntaub ntawv tseem ceeb xws li qis - cov noob ntau yam sib txawv thiab cov kab sib txuas;
Kev txheeb xyuas ntau-dimensional
Kev sib koom ua ke ntau- cov ntaub ntawv qib xws li genome thiab cov ntaub ntawv sau tseg, muab kev tshuaj xyuas dav dav los ntawm qhov ntev ntawm cov yam ntxwv ntawm caj ces, cov khoom ua haujlwm, thiab kev pheej hmoo ntawm kev nyab xeeb;
Cov txheej txheem txheem
nruj me ntsis raws li kev soj ntsuam zoo cov qauv, cov txheej txheem tsom xam cov ntaub ntawv yog traceable thiab repeatable, xyuas kom meej qhov tseeb thiab kev ntseeg siab ntawm cov kev tshwm sim;
Cov ntsiab lus kuaj
• Lub luag haujlwm tseem ceeb: Los ntawm kev txheeb xyuas cov kab ntawm tes- tshwj xeeb luv luv tandem rov ua dua (STR) ib ntus thiab muab piv nrog cov txheej txheem thoob ntiaj teb cov ntaub ntawv (xws li ATCC thiab DSMZ), qhov tseeb ntawm kab xov tooj ntawm tes tuaj yeem lees paub;
• Kev tshawb pom qhov zoo: Kev paub qhov tseeb ntawm lub hauv paus ntawm cov kab ntawm tes thiab tsis txhob hla -kev sib kis (xws li kev tsis meej pem ntawm tib neeg thiab cov kab xov tooj ntawm tes) yog qhov tseem ceeb lav rau cov ntaub ntawv kev ntseeg siab thiab kev ua raws li kev lag luam;
Cov haujlwm tseem ceeb: Txheeb xyuas qhov chaw nkag, luam tus lej, thiab kev ua tiav ntawm lub hom phiaj ntawm cov noob caj noob ces (xws li recombinant protein qhia cell kab thiab gene kho cov kab ntawm tes);
• Kev tshawb nrhiav noob caj noob ces: Kev tshuaj ntsuam xyuas ntawm cov caj ces txawv txav xws li cov noob hloov pauv (SNV), cov lej hloov pauv (CNV), thiab cov qauv kev hloov pauv (SV) hauv cov kab ntawm tes, thiab kev tshuaj xyuas ntawm kev sib koom ntawm cov kab ntawm tes phenotype thiab cov yam ntxwv ntawm caj ces;
• Kev tshuaj xyuas Chromosomal kev ncaj ncees: Tshawb xyuas cov qauv kev hloov pauv xws li tus lej chromosome txawv txav, hloov chaw, thiab tshem tawm, thiab ntsuas cov caj ces ruaj khov ntawm cov kab ntawm tes;
• Kev tsom xam cov noob muaj nuj nqi: Txheeb xyuas cov yam ntxwv ntawm kev qhia thiab cov txheej txheem tswj hwm ntawm cov noob muaj feem xyuam rau cov tub ntxhais ntawm cov kab ua haujlwm (xws li kev loj hlob, kev sib txawv, thiab cov metabolism hauv cov noob) los muab cov kev taw qhia rau cov kab cell optimization.
Hom kab ntawm tesnpog
Engineered cell kab
recombinant protein qhia cell kab (CHO, HEK293), gene kho cell kab (CRISPR/Cas9 hloov hlwb), CAR engineered cell kab, thiab lwm yam .;
Stem cell kab
Kev tshuaj ntsuam genetic stability thiab sib txawv muaj peev xwm ntawm embryonic qia hlwb (ESC), induced pluripotent qia hlwb (iPSC), mesenchymal qia hlwb (MSC);
Cov kab mob niaj hnub
Kev txheeb xyuas, kev kuaj kab mob sib kis, thiab kev txheeb xyuas tus yam ntxwv ntawm cov kab ke niaj hnub sim siv hauv kev tshawb fawb (xws li HeLa, A549, MCF-7).
Daim ntawv thov Scenario
Kev tshawb fawb theem pib
cell kab authentication, genetic yam ntxwv tsom xam, thiab kev sim raws tu qauv optimization kom paub tseeb tias qhov tseeb thiab reproducibility ntawm cov ntaub ntawv;
Biopharmaceutical R & D
Recombinant cell kab kev tsim kho thiab kev tshuaj ntsuam xyuas, lub hom phiaj gene insertion verification, thiab cell kab stability xyuas, pab rau kev tsim cov tshuaj protein thiab tshuaj tiv thaiv kab mob;
Hauv kev kho mob ntawm tes
ntsuam xyuas cov kab mob genetic stability ntawm stem cell kab thiab engineered cell kab, txheeb xyuas cov kev pheej hmoo ntawm kev nyab xeeb, thiab muab kev txhawb nqa kev ua raws li kev siv tshuaj kho mob;
Kev tswj hwm theem zoo
Kev soj ntsuam kev ruaj ntseg, kev txheeb xyuas tus kheej sib xws, thiab kev tshawb xyuas kev sib kis thaum lub sij hawm xov tooj ntawm tes hla los xyuas kom meej tias kev ua raws li cov txheej txheem tsim khoom.