Cov ntsiab lus tseem ceeb tsim ntawm plaub txoj kev

1. Bioassay

Txheeb xyuas cov hom phiaj xws li cov kab mob antigens thiab cov proteins tshwj xeeb.

Ua kom zoo rau cov tshuaj tiv thaiv kab mob thiab teeb tsa cov kab sib txawv thiab qhov kev txwv tsawg kawg nkaus.

2. qPCR (qPCR)

Tsim tshwj xeeb primers/probes kom tsis txhob hla -reactions

Kho qhov kev pab cuam cov tshuaj tiv thaiv, suav nrog annealing kub thiab ncua sij hawm.

Kev tshuaj ntsuam xyuas cov noob hauv nruab nrab kom ntseeg tau qhov tseeb ntawm cov ntaub ntawv normalization

3. ELISA (Enzyme-Txuas Kev Ntsuas Immunosorbent)

Kev tshuaj ntsuam xyuas cov tshuaj tiv thaiv kab mob tshwj xeeb (kev ntes + kuaj pom cov tshuaj tiv thaiv)

Optimize txheej concentration, thaiv kev daws, thiab incubation lub sij hawm tsis.

4. Cell- raws li txoj kev tshawb nrhiav

Xaiv cov cell uas tsim nyog thiab txiav txim siab txog qhov ntom ntom thiab kab lis kev cai.

Tsim cov txheej txheem txhais lus xws li fluorescence intensity threshold thiab cytopathic effect score.

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Optimize txheej txheem

 

Los ntawm kev siv ib qho -factor variable method orthogonal trials, key parameters such as react temperature and reagent dosage is erratively tested. Los ntawm kev kaw- voj optimization ntawm "kev hloov pauv parameter - kev txheeb xyuas qhov tshwm sim - kev ntsuas kev sib txawv", qhov tseeb, qhov tseeb thiab qhov muaj zog ntawm txoj kev tau txhim kho.

 

Core Zoo

Npog plaub hom kev tshawb nrhiav thiab hloov mus rau ntau yam kev kuaj lom

Customized kev daws teeb meem kom tau raws li qhov sib txawv ntawm qhov kev ntsuas qhov tseeb

Kaw- txheej txheem kev ua kom zoo dua ua kom muaj kev ruaj ntseg thiab kev ntseeg tau ntawm txoj kev.

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